Blood Cells by Bain B.J.

April 3, 2017 | Clinical Chemistry | By admin | 0 Comments

By Bain B.J.

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New methods for the estimation of haemoglobin concentration have been introduced speciically for near‐ patient testing (see below). Recommended units The ICSH has recommended that Hb be expressed as g/l (mass concentration), or as mmol/l (molar concentration) in terms of concentration of the haemoglobin monomer. e. 45 mmol/l. If Hb is expressed in molar concentration, then MCH and MCHC should also be expressed in this manner. 70 fmol. Similarly, an MCHC of 330 g/l (33 g/dl) is equivalent to 20 mmol/l.

Particles greater than 35 l after red cell lysis are counted as white cells. The instrument is able to count NRBC and corrects the WBC for NRBC interference [53]. Platelets are counted between 2 and 20 l, but the curve is extrapolated to 70 l to include large platelets. Reticulocytes can be counted in a separate mode (see below). A red cell variable, the mean sphered cell volume (MSCV), an artiicial measurement in the reticulocyte channel, represents the average volume of sphered red cells in hypo‐osmotic conditions [54].

In the former case the uncorrected count is a TNCC rather than a WBC, and this is used for calculating absolute cell numbers. In the latter case, the TNCC 24 Chapter 2 is corrected to a WBC by subtracting the number of NRBC. Laboratories using automated instruments that produce a TNCC rather than a WBC should consistently follow one or other convention of expressing counts. It is probably better not to correct the TNCC to a WBC, but to calculate absolute counts from the TNCC and the percentage of each cell type, NRBC being included in the differential count.

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