By Bain B.J.
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Extra resources for Blood Cells
New methods for the estimation of haemoglobin concentration have been introduced speciically for near‐ patient testing (see below). Recommended units The ICSH has recommended that Hb be expressed as g/l (mass concentration), or as mmol/l (molar concentration) in terms of concentration of the haemoglobin monomer. e. 45 mmol/l. If Hb is expressed in molar concentration, then MCH and MCHC should also be expressed in this manner. 70 fmol. Similarly, an MCHC of 330 g/l (33 g/dl) is equivalent to 20 mmol/l.
Particles greater than 35 l after red cell lysis are counted as white cells. The instrument is able to count NRBC and corrects the WBC for NRBC interference . Platelets are counted between 2 and 20 l, but the curve is extrapolated to 70 l to include large platelets. Reticulocytes can be counted in a separate mode (see below). A red cell variable, the mean sphered cell volume (MSCV), an artiicial measurement in the reticulocyte channel, represents the average volume of sphered red cells in hypo‐osmotic conditions .
In the former case the uncorrected count is a TNCC rather than a WBC, and this is used for calculating absolute cell numbers. In the latter case, the TNCC 24 Chapter 2 is corrected to a WBC by subtracting the number of NRBC. Laboratories using automated instruments that produce a TNCC rather than a WBC should consistently follow one or other convention of expressing counts. It is probably better not to correct the TNCC to a WBC, but to calculate absolute counts from the TNCC and the percentage of each cell type, NRBC being included in the differential count.